![]() Optogenetic tools and fluorescent biosensors for calcium imaging have made it possible to record and control brain activity during such behavioral tasks to identify neural circuits involved in overt behavior ( Bernstein and Boyden, 2011 Knopfel, 2012). By controlling the presentation of stimuli and their relationship to the responses of test subjects, experimenters can design behavioral tasks that assess motivation, learning (operant and classical conditioning) or other cognitive processes (e.g., attention, inhibitory control, etc.). An operant chamber allows for precisely controlled presentation of stimuli (auditory, visual, etc.) and for the measurement of responses to these stimuli (e.g., lever presses or nose pokes). Operant conditioning chambers (“Skinner boxes”) are widely used in animal research to study learning and other cognitive processes in rodents, some birds, and nonhuman primates ( Panlilio and Goldberg, 2007 Paglieri et al., 2014). We propose that our operant chamber is a cheaper alternative to its commercially available counterparts and offers a better balance between versatility and user-friendly setup than other open-source alternatives. We also present calcium imaging data of medial prefrontal cortex (mPFC) neuronal activity acquired during Go/No-Go testing in freely moving mice and show that neuronal population activity increases from day 1 to day 14 of the task. Using this setup, we replicate our previously published findings showing that mice exposed to social defeat stress in adolescence have inhibitory control impairments in the Go/No-Go task when they reach adulthood. The advantages of our operant setup compared with other open-source and closed-source solutions are its relatively low cost, its support of complex behavioral tasks, its user-friendly setup, and its validated functionality with video imaging of behavior and calcium imaging using the UCLA Miniscope. Here, we present a custom-built operant chamber that can be constructed in a few days by an unexperienced user with relatively inexpensive, widely available materials. The behavioral testing and imaging setups that are commercially available are often quite costly. Paired with the rapidly developing technologies for brain imaging and manipulations of brain activity, operant conditioning chambers are a powerful tool for neuroscience research. It doesn’t matter if you’re live streaming from your phone or computer, these tips can help you become a broadcaster and presenter.Operant chambers are widely used in animal research to study cognition, motivation, and learning processes. It allows you to invite more people locally or from all over the world, depending on your industry. ![]() Streaming your live event is a great way to extend your event capacity and maximize your efforts. Here are other blog posts that you might like:ĥ Tips to Live Stream your Event Like a Pro We’ll soon be updating our website with more “How To” videos to show you how to use the YouTube features and the mobile app. ![]() ![]() Learn more about the Man圜am app available for iOS and Android. The Man圜am Mobile Source for Android is free and available for download on Google Play. Connecting to Man圜am with your phone is easy, simply enter the 4 digit code that pops up when you open the app, or use a QR code scanner, and you are ready to roll! We are also very excited to announce the release of the Man圜am Mobile Source App for Android! With the mobile app, you can record live videos on the go and stream directly to Man圜am using your phone. Also, we’ve added YouTube as one of the “Share” options, so you can record your own videos and upload them to YouTube directly from Man圜am, In this update, we’ve added YouTube as a video source option, where you can stream and broadcast your favorite videos found on YouTube more easily, just by entering the video URL. This new version includes several awesome new features such as YouTube integration and mobile capabilities. We are pleased to announce that our newest update, Man圜am 4.0.97 is officially available for download. (Check out Man圜am 6, the newest version: What you need to know about the new Manycam 6 )
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